Selig, L. et al.

Helices (A351–V378) from two neighbouring subunits interact with each other and are critical for tetramer formation. 7-deaza-dGTP (7-deaza-2´-deoxyguanosine 5´-triphosphate) contains a 5 mM solution of 7-deaza-dGTP as a dilithium salt. Mutations of dGTP binding residues in the allosteric site affect tetramer formation, dNTPase activity and HIV-1 restriction. You can also search for this author in Vorontsov, I. I. et al. SAMHD1 is the dendritic- and myeloid-cell-specific HIV-1 restriction factor counteracted by Vpx. Nature Communications You can also search for this author in Vpx relieves inhibition of HIV-1 infection of macrophages mediated by the SAMHD1 protein. We have co-crystallized SAMHD1c′ with its dGTP/dATP substrates and solved the structure by the molecular replacement method, using 3U1N (SAMHD1c) as … Yu, X. F., Yu, Q. C., Essex, M. & Lee, T. H. The vpx gene of simian immunodeficiency virus facilitates efficient viral replication in fresh lymphocytes and macrophage.

Tetramerization of SAMHD1 is required for biological activity and inhibition of HIV infection. dGTP-triggered tetramer formation is also important for SAMHD1-mediated LINE-1 regulation.

However, the mechanism behind dGTP selectivity is unclear. Draw the structure of dGTP? However, unlike The dGTP-induced dNTPase activity was much higher for the tetramer than for the dimer (The dNTPase activity of SAMHD1 has been linked to HIV-1 restrictionExamination of the SAMHD1 tetramer also revealed interesting information about amino acid mutations reported in AGS patients. Adams, P. D. et al. Deoxyuridine triphosphate (dUTP) is a deoxynucleotide triphosphate that is chemically similar to uridine triphosphate except that it has a deoxyribose sugar instead of a ribose sugar.DNA synthesis requires the availability of deoxynucleotide triphosphates (dTTP, dATP, dGTP, dCTP), whereas RNA synthesis requires the availability of nucleotide triphosphates (NTPs) such as TTP, ATP, GTP, and UTP. Laskowski, R., Macarthur, M., Moss, D. & Thornton, J. Procheck—A program to check the stereochemical quality of protein structures. These mutations were created to destroy hydrogen bonding with the dGTP/dATP molecules in the allosteric sites of SAMHD1. Mutations involved in Aicardi-Goutieres syndrome implicate SAMHD1 as regulator of the innate immune response. Accola, M. A., Bukovsky, A. Moran, J. V. et al. Epub 2020 Jun 8.Viruses. Epub 2020 Jan 21.Li Z, Huan C, Wang H, Liu Y, Liu X, Su X, Yu J, Zhao Z, Yu XF, Zheng B, Zhang W.EMBO Rep. 2020 Jan 7;21(1):e47528. SAMHD1c′Q142E/R145K, SAMHD1c′R333E/R451E, SAMHD1c′R333E, SAMHD1c′R451E and SAMHD1c′D137A, all had a reduced ability to form tetramers when compared with SAMHD1c′ (The tetrameric structure of SAMHD1 reported here is significantly different from the published SAMHD1 dimer (Another functional property of SAMHD1 is its sensitivity to Vpx-mediated degradationWe have recently observed that the human SAMHD1 protein is a potent suppressor of LINE-1 activityIt has been reported that a mouse LINE-1 assay system could produce a more efficient retrotransposition event in HEK293T cellsSAMHD1 is a dGTP-activated dNTPase. The mechanism of its dGTP-activated dNTPase function remains unclear. Restricting HIV the SAMHD1 way: through nucleotide starvation.

An improved plasmid DNA expression vector for direct injection into skeletal muscle. Mutations of dGTP binding residues in the allosteric site affect tetramer formation, dNTPase activity and HIV-1 restriction. Supplied in: Milli-Q® water as a lithium salt at (pH 7.0). SAMHD1-deficient CD14+ cells from individuals with Aicardi-Goutieres syndrome are highly susceptible to HIV-1 infection. A. Zhao, K. et al. Hofmann, H. et al. 2019 Aug 30;47(4):1013-1027. doi: 10.1042/BST20180348. Recent data also indicate that SAMHD1 regulates retrotransposition of LINE-1 elements. SAMHD1 is a dGTP-activated deoxynucleoside triphosphate triphosphohydrolase (dNTPase) whose dNTPase activity has been linked to HIV/SIV restriction.

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